6/15/2023 0 Comments Insert scale bar imagej![]() Using the rectangle selection tool, create a box around the area you’d like to crop, then click Add or press “t” again to add that selection to the manager. The ROI Manager allows you to work with multiple selections from different parts of a single image or multiple images. To make a crop of a particular region of interest (ROI), open the ROI Manager using Analyze -> Tools -> ROI Manager or the shortcut “t”. It’s generally best to use the same crop size for all images in a figure panel to ensure you maintain the scale of cells you are comparing. This is especially important when working with large-format montage images like the ones shown. Cropping is often necessary to show close details like intracellular structure or cut out any erroneous parts of the image. The next thing we want to do is make a crop of the particular cells or part of our images that we’d like to show. Our lab primarily uses Nikon microscope systems, therefore, we use NIS-Elements Viewer to quickly scan through images. I prefer the added plugins that come pre-installed with Fiji, download the latest version from: Īdobe Illustrator or any other vector graphics editorĪ raw image viewing software is optional. ![]() With that out of the way, let’s get started! Requirements I want to stress that there are many many ways to skin this cat, as is the case for just about everything in science. Furthermore, this post was primarily written to assist new students in our own lab, and is therefore largely tailored to our own setup and systems. However, these methods are by no means all-encompassing and are certainly not the only correct way of editing images for publication or presentation. The methods I describe below were taught to me by several excellent researchers. This is cause for concern given that post-processing image manipulation needs to be done in a manner that ensures authenticity and does not misrepresent the underlying image data. A recent survey revealed that the quality of reporting of image capturing and processing methodologies is unacceptably low in biomedical research articles, where key information and details are largely absent. Given the breadth of options available in microscope systems, image analysis and visualisation platforms, and graphic design software, the number of choices can be overwhelming and succinct start-to-finish tutorials hard to find. Creating visually appealing, understandable and scientifically accurate image-based figures can be challenging.
0 Comments
Leave a Reply. |